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Yeast protocols, molecular biology protocols, media and general protocols used by the Cell Cycle Group
     Yeast Protocols | Molecular Biology Protocols | Media | General Protocols

Genomic DNA Mini-prep
Adapted for Fastprep machine


Lysis buffer
– 100 mM Tris pH 8.0
– 50 mM EDTA
– 1% SDS
For 50 ml: 5 ml 1 M Tris, 5 ml 0.5 M EDTA, 5 ml 10% SDS


  1. Grow 5 ml cells overnight at 30° C.
  2. Spin, wash once with 1 ml H2O.
  3. Resuspend in 500 µl lysis buffer.
  4. Transfer to a screw-tube with acid washed glass beads.
  5. Fastprep at 6.0 speed for 20 min.
  6. Recover liquid phase with blue tip into another tube.
  7. Add 275 µl 7 M ammonium acetate pH 7.0.
  8. Incubate 5 min at 65° C, then 5 min on ice.
  9. Add 500 µl chloroform, vortex, spin 2 min in microfuge.
  10. Take supernatant and precipitate with 1 ml isopropanol.
  11. Incubate 5 min at room temperature, then spin 5 min.
  12. Wash pellet with 70% EtOH, dry and dissolve in 50 µl H2O.
For Southern, digest 5 µl DNA.
For PCR, use 0.5-1 µl DNA.